35848
Sigma
2′,7′-Dichlorofluorescein
Standard Fluka, fluorescence indicator, ≥90% (T)
| CAS Number: | 76-54-0 |
| Linear Formula: | C20H10Cl2O5 |
| Molecular Weight: | 401.20 |
| Beilstein Registry Number: | 58009 |
| EC Number: | 200-968-6 |
| MDL number: | MFCD00005047 |
| PubChem Substance ID: | 24861978 |
Description
| Application | Application example: Dip reagent for the fluorimetric determination of vicinal diols, glycosides and phenols. Solution A: Saturated solution of lead tetraacetate (~2%) (Fluka-No. 15370) in glacial acetic acid Solution B: 2',7'-Dichlorofluorescein in ethanol (0.2-1%) Mix 5 mL solution A with 5 mL solution B and bring to 200 mL with toluene, just prior to using. Solution should be freshly prepared each time. Dipping time: 10 sec. |
| Other usage examples: determination of carbohydrates by fluorescence densitometry after TLC1; determination of H2O2 by chemiluminescence2,3; fluorescent additive used in indirect fluorimetric detection in OTC-HPLC4 |
Properties
| grade | fluorescence indicator |
| Standard Fluka | |
| assay | ≥90% (T) |
| fluorescence | λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0 |
| mp | 280 °C (dec.)(lit.) |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
References
| Cited Reference | 1. G. Gübitz et al. J. Chromatogr. 117, 337, (1976) Abstract |
| 2. H. Watanabe et al. Anal. Sci. 2, 461, (1986) | |
| 3. T. Segawa et al. Anal. Sci. 6, 763, (1990) | |
| 4. W.D. Pfeffer, E.S. Yeung J. Chromatogr. 506, 401, (1990) | |
| Beilstein | Beil. 19,V,6,461 |
| reference | FT-IR 2 (3), 4148:A / FT-IR 1 (2), 1010:D / IR-Spectra (3), 1473:D / RegBook 1 (2), 2793:L / Sigma FT-IR 1 (2), 125:A / Stains and Dyes Ref, 251 / Structure Index 1, 444:A:6 |
